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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/33538

Title: Genome-scale Metabolic Network Reconstruction and Constraint-based Flux Balance Analysis of Toxoplasma gondii
Authors: Song, Carl Yulun
Advisor: Parkinson, John
Department: Molecular and Medical Genetics
Keywords: Flux balance analysis
Toxoplasma gondii
Metabolic network reconstruction
in silico metabolism
Issue Date: 27-Nov-2012
Abstract: The increasing prevalence of apicomplexan parasites such as Plasmodium, Toxoplasma, and Cryptosporidium represents a significant global healthcare burden. Treatment options are increasingly limited due to the emergence of new resistant strains. We postulate that parasites have evolved distinct metabolic strategies critical for growth and survival during human infections, and therefore susceptible to drug targeting using a systematic approach. I developed iCS306, a fully characterized metabolic network reconstruction of the model organism Toxoplasma gondii via extensive curation of available genomic and biochemical data. Using available microarray data, metabolic constraints for six different clinical strains of Toxoplasma were modeled. I conducted various in silico experiments using flux balance analysis in order to identify essential metabolic processes, and to illustrate the differences in metabolic behaviour across Toxoplasma strains. The results elucidate probable explanations for the underlying mechanisms which account for the similarities and differences among strains of Toxoplasma, and among species of Apicomplexa.
URI: http://hdl.handle.net/1807/33538
Appears in Collections:Master

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