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|Title: ||Synthesis of a Novel Acyl Phosphate Cross-linker and its Modification of Hemoglobin|
|Authors: ||Wilson, Elizabeth|
|Advisor: ||Kluger, Ronald|
|Issue Date: ||27-Nov-2012|
|Abstract: ||Hemoglobin-based oxygen carriers (HBOCs) are of great interest for their potential as
a safer alternative to blood transfusions. To overcome the vasoactivity associated with
small HBOCs, our group is interested in connecting two hemoglobin tetramers together,
forming “bis-tetramers”. Bis-tetramers have previously been synthesized by our group,
but yield and purity of the resulting solutions have been low and hindered their usefulness for trials. A new cross-linker was designed in an attempt to improve yield. This thesis describes the synthesis of an acyl phosphate cross-linker N-[bis(sodium methyl phosphate)isophthalyl]-4-azidomethylbenzoate (5), its modification of hemoglobin and subsequent purification attempts of the resulting solution. Cross-linker 5 was found to be selective to β-β-crosslinking and produced singly modified subunits as byproducts.
Attempts to purify the resulting reaction mixture by heating resulted in the decomposition of the azide group on the cross-linker, which was critical for the coupling step. Efforts to overcome this problem were unsuccessful.|
|Appears in Collections:||Master|
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