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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/3497

Title: Ca2+ and reactive oxygen species are involved in the defense responses of rice callus culture to rice blast disease
Authors: Kuta, D. D.
Gaivaronskaya, L. M.
Keywords: Biotechnology
Oryza sativa, Magnaporthe grisea, hypersensitive response, reactive oxygen species, calcium. jb04013
Issue Date: Jan-2004
Publisher: Academic Journals
Citation: African Journal of Biotechnology 3(1)
Abstract: The role of Ca2+ and reactive oxygen species in the defense responses of callus cultures of rice (Oryza sativa L., cv. Zenith) to infection with avirulent strain of rice blast fungus (Magnaporthe grisea, strain Ina168) was investigated. It was observed that rice calli, especially after mild blast infection, exude substances (diffusate) that inhibit spore germination of the avirulent blast fungus. This fungitoxic calli diffusate led to superoxide dismutase-sensitive reduction of Nitro-blue-tetrazolium. Treatment of rice calli with crude elicitor from the blast fungus also led to hypersensitive necrotic response. Addition of antioxidant reagents diminishes the necrotic response of calli to the elictor treatment, implicating the involvement of reactive oxygen species in the hypersensitive necrotic response. When ethyleneglycoltetraacetic acid (Ca2+ chelator) or LaCl3 (Ca2+channel blocker) was added, the necrotic response of calli to elicitor treatment was also significantly weakened, implying the involvement of Ca2+ in the defense response.
URI: http://bioline.utsc.utoronto.ca/archive/00002350/01/jb04013.pdf
http://hdl.handle.net/1807/3497
Other Identifiers: http://bioline.utsc.utoronto.ca/archive/00002350/
Appears in Collections:Bioline International Legacy Collection

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