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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/4161

Title: RESEARCH ARTICLE - Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart
Authors: Ojalvo, Ariana G.
Seralena, Alina
Vispo, Nelson S.
Silva, Ricardo
Gonzalez, Noel
Guevara, Luis
Batista, Juan F.
Montequin, Jose F.
Chaos, Nicolas
González, Rafael
Reima, Camilo
Peña, Yamile
Coca, Marcos
Perera, Alejandro
Vazquez, Raysa
Puchades, Yaquelin
Garcia-Osuna, Tamara
Dominguez, Heberto
Reyes, Jose L.
Ali, Alfonso
Herrera, Luis
Keywords: Biotechnology
collateral development, coronary artery disease, gene therapy, naked plasmid DNA, revascularization, therapeutic angiogenesis. ej04030
Issue Date: Dec-2004
Publisher: Universidad Católica de Valparaíso
Citation: Electronic Journal of Biotechnology 7(3)
Abstract: Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is angiogenic in vitro and in vivo. Several studies report on gene transfer of VEGF121 to promote angiogenesis in the ischemic myocardium of animals and patients. We hypothesized that intramyocardial administration of naked plasmid DNA encoding VEGF121 could improve myocardial perfusion and function in a porcine model of myocardial ischemia. Yorkshire swine underwent thoracotomy and placement of an ameroid constrictor on the circumflex coronary artery. Four weeks later, pVEGF121 plasmid was administered into the ischemic myocardium. Four weeks after gene transfer, SPECT imaging demonstrated significant reduction in the ischemic area in pVEGF121-treated animals compared with controls. In the pVEGF121 group, most of the animals evolved from light ischemia to a normal perfusion. In contrast, control animals exhibited similar or impaired ischemic conditions. Our results indicate that intramyocardial gene transfer of VEGF121 as naked plasmid DNA results in significant improvement in myocardial perfusion and function.
URI: http://bioline.utsc.utoronto.ca/archive/00003040/01/ej04030.pdf
Other Identifiers: http://bioline.utsc.utoronto.ca/archive/00003040/
Appears in Collections:Bioline International Legacy Collection

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