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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/4221

Title: DURACIÓN DEL EFECTO REVIGORIZANTE DE PODAS SEVERAS DE PLANTAS ADULTAS DE AVELLANO EUROPEO (Corylus avellana L.) cv. Negretta SOBRE EL CULTIVO IN VITRO Duration of the reinvigorating effect of severe pruning of mature European hazelnut plants (Corylus avellana L.) cv. Negretta with in vitro cultivation
Authors: Sánchez-Olate, Manuel
Ríos, Darcy
Rodríguez, Roberto
Materán, María Elena
Pereira, Guillermo
Keywords: Plant Sciences
hazelnut, in vitro culture, morphogenetic capacity, mature tissue. avellano, cultivo in vitro, capacidad morfogénica, tejido adulto. at04039
Issue Date: Oct-2004
Publisher: Instituto de Investigaciones Agropecuarias, INIA
Citation: Agricultura Técnica 64(4)
Abstract: The duration of the reinvigorating effect of severe pruning on mature European hazelnuts (Corylus avellana L.) cv. Negretta was analyzed. The in vitro stimulation and proliferation rate of the resulting explants from cutting stimulation obtained from adult plants not pruned (T1), epicormic shoots of non-pruned plants (T2), once-pruned plants (T3) and plants pruned twice (T4) were evaluated. In a first stage, the response to climatic stimulation was evaluated using shoots between 25 and 35 cm length and basal diameters between 0.5 and 1.5 cm, obtained from T1, T2, T3 and T4 plants. In a second stage, the proliferation rate of in vitro explants generated by the climatic stimulation was evaluated. Results were analyzed by ANOVA using a completely random design. The positive effect of continuous severe pruning on the morphogenic capacity was confirmed, being significantly larger for T2 and T4 (100 and 87.5% stimulation, respectively). The in vitro multiplication rate was significantly larger in T2 and T4 (3.9 and 3.2 new shoots per explant, respectively), showing that epicormic shoots and severe pruning every two years are alternatives to obtain adequate plant material for in vitro multiplication of this species. It was also established that severe pruning does not significantly affect the physiology and development of explants, and that the reinvigorating effect of severe pruning has a duration near to two years. It is necessary to consider this treatment as a fundamental technique to keep the species vigorous. RESUMEN Se analizó la duración del efecto revigorizante de podas severas de avellano europeo (Corylus avellana L.) cv. Negretta adulto. Se evaluó la estimulación y la tasa de proliferación in vitro, sobre explantos resultantes de la estimulación de varetas obtenidas desde plantas adultas sin poda (T1), brotes epicórmicos de plantas sin poda (T2), plantas podadas una sola vez (T3) y plantas podadas dos veces (T4). En una primera etapa, se evaluó la respuesta a la estimulación climática utilizando varetas de entre 25 y 35 cm de largo y diámetros basales de entre 0,5 y 1,5 cm, obtenidas de plantas T1, T2, T3 y T4. En una segunda etapa, se evaluó la tasa de proliferación in vitro de explantos originados por la estimulación climática. Los resultados se analizaron mediante ANDEVA, utilizando un diseño completamente aleatorio. Se confirmó el efecto positivo de podas severas continuas sobre la capacidad morfogénica, siendo significativamente mayores para T2 y T4 (100 y 87,5% de estimulación, respectivamente). Las tasas de multiplicación in vitro fueron significativamente mayores en T2 y T4 (3,9 y 3,2 tallos nuevos por explanto, respectivamente), dejando de manifiesto que los brotes epicórmicos y podas severas cada dos años, son alternativas para la obtención de material vegetal adecuado para la multiplicación in vitro de esta especie. Además, se estableció que podar severamente no afecta significativamente la fisiología y desarrollo de explantos, y que el efecto revigorizante de las podas severas tiene una duración cercana a los dos años, siendo necesario considerar este tratamiento como una técnica fundamental para mantener el vigor de la especie.
URI: http://bioline.utsc.utoronto.ca/archive/00003110/01/at04039.pdf
Other Identifiers: http://bioline.utsc.utoronto.ca/archive/00003110/
Appears in Collections:Bioline International Legacy Collection

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