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|Title: ||Developmental analysis of factors binding to the mouse 68-kDa neurofilament promoter|
|Authors: ||Kure, Robert|
Brown, Ian R.
|Keywords: ||Intermediate filament ; gene expression ; transcription ; neuron|
|Issue Date: ||1997|
|Publisher: ||Kluwer Academic/Plenum Publishers|
|Citation: ||Neurochemical Research, Vol. 22. No. 5, 1997, pp. 555-562|
|Abstract: ||Whole tissue extracts prepared from mouse brain regions at various postnatal ages were characterized for binding of factors to the DNase I hypersensitive site (HSS1) which is located closest to the transcription start site of the 68-kDa mouse neurofilament gene (NF-L). Gel mobility shift assays detected changes in factor binding during postnatal development of the neocortex. Competition experiments suggested that one of the complexes resulted from factor binding to a 9 bp sequence found in both the light and medium neurofilament promoter regions (NF-L/M). Gel mobility shifts performed with an oligonucleotide probe containing the NF-L/M sequence detected two brain-specific DNA-protein complexes, and a third complex in both brain and liver. During cerebellar and neocortical development, one of the NF-L/M complexes was most intense at postnatal day 10 when transcription of the NF-L gene is upregulated.|
|Appears in Collections:||Biology|
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