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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/4281

Title: Developmental analysis of factors binding to the mouse 68-kDa neurofilament promoter
Authors: Kure, Robert
Brown, Ian R.
Keywords: Intermediate filament ; gene expression ; transcription ; neuron
Issue Date: 1997
Publisher: Kluwer Academic/Plenum Publishers
Citation: Neurochemical Research, Vol. 22. No. 5, 1997, pp. 555-562
Abstract: Whole tissue extracts prepared from mouse brain regions at various postnatal ages were characterized for binding of factors to the DNase I hypersensitive site (HSS1) which is located closest to the transcription start site of the 68-kDa mouse neurofilament gene (NF-L). Gel mobility shift assays detected changes in factor binding during postnatal development of the neocortex. Competition experiments suggested that one of the complexes resulted from factor binding to a 9 bp sequence found in both the light and medium neurofilament promoter regions (NF-L/M). Gel mobility shifts performed with an oligonucleotide probe containing the NF-L/M sequence detected two brain-specific DNA-protein complexes, and a third complex in both brain and liver. During cerebellar and neocortical development, one of the NF-L/M complexes was most intense at postnatal day 10 when transcription of the NF-L gene is upregulated.
URI: http://hdl.handle.net/1807/4281
Appears in Collections:Biology

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