|Title: ||Synergistic interaction between two linear inhibitors on a single
enzyme: Vanadate and L-phenylalanine inhibition of rat liver alkaline
|Authors: ||Malomo, Sylvia O.|
Olorunniji, Femi J.
Arise, Rotimi O.
Adebayo, Joseph O.
Adedsu, Olaniyi T.
Odutuga, Adewale A.
|Keywords: ||alkaline phosphatase, synergistic inhibition|
|Issue Date: ||31-Dec-2003|
|Publisher: ||Nigerian Society for Experimental Biology|
|Citation: ||Biokemistri (ISSN: 0795-8080) Vol 13 Num 1|
|Abstract: ||The combined effect of two linear inhibitors of rat liver alkaline
phosphatase (ALP). vanadate (Van) and L-phenylalanine (L-phe) were
studied using a modification of the common Yonetani-Theorell procedure
proposed for studying synergistic inhibition. The modes of inhibition
of ALP by Van and L-phe as analysed using the double reciprocal plots
of the Michaelis-Menten equation were mixed and uncompetitive
inhibition respectively. Analysis of the combined effect of the two
inhibitors showed that their inhibitory effects were mutually enhanced.
The mechanistic aspects and practical applications of the procedure are
|Other Identifiers: ||http://www.bioline.org.br/abstract?id=bk03004|
|Rights: ||Copyright 2003 - Nigerian Society for Experimental Biology|
|Appears in Collections:||Bioline International Legacy Collection|