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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/5220

Title: Radiosensibility of two cowpea (Vigna unguiculata (L.) Walp) genotypes to gamma irradiations
Authors: Lemus, Yrasema
Méndez-Natera, Jesús Rafael
Cedeño, Jesús Rafael
Otahola-Gómez, Víctor
Keywords: Cowpea, Vigna unguiculata, gamma rays, irradiation
Frijol, Vigna unguiculata, rayos gamma, irradiación
Issue Date: 31-Dec-2002
Publisher: Universidad de Oriente Press
Citation: Revista Científica UDO Agrícola (ISSN: 1317-9152) Vol 2 Num 1
Abstract: The use of induced mutations in the genetic improvement of plants has allowed the obtaining of cultivars of high adaptation in many species. However, their use should be preceded of experiments that allow to determine the appropriate dosimetry, as well as to familiarize to the plant breeder with the effect that radiations have on the used vegetable material. With this objective, it was carried out a experiment at glasshouse of Postgraduate of the Nucleo de Monagas of the Universidad de Oriente, using seeds of two cowpea cultivars (TC 9-6 and A-4), which were irradiated with five dose of gamma rays (20; 40; 60; 80 and 100 Krad), compared with a control without irradiation. The seeds were irradiated in the reactor of 60Co of the Instituto Venezolano de Investigaciones Científicas (IVIC) in Caracas and sowed 5 days after the irradiation, using aluminium trays of 45 cm of high, 30 cm of wide and 15 cm of depth. Washed sand was used as substrate. A 2 × 5 factorial experiment in a randomized complete block design was used with four replications. The Duncan´s multiple range test was used to detect the differences among treatment. The level of 5 % of signification was used. The evaluated parameters were: plant survival, mean number of days to the total germination, plant height and number of leaves at 7 and 21 days after the sowing, aerial part dry weight and radicle dry weight. Significant interaction was observed between the genotypes and irradiation doses in all characters, excepting mean number of days for complete germination and for radicle dry weight, where differences were only observed for simple effect of the studied factors. When analyzing the data and to compare them with the treatments of seeds without irradiation, it was observed that in all the evaluated characters, the 50 lethal dosis (LD50) or 50 reductive (GR50) were between 20 and 60 Krad. In the other hand, there were varietal differences to the radiation, being cultivar TC-9-6 more resistant than A-4.
URI: http://hdl.handle.net/1807/5220
Other Identifiers: http://www.bioline.org.br/abstract?id=cg02004
Rights: © Copyright 2002 - Revista Científica UDO Agrícola
Appears in Collections:Bioline International Legacy Collection

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