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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/6503

Title: Full Length Research Paper - Effect of nitrates on embryo induction efficiency in cotton (Gossypium hirsutum L.)
Authors: Ikram-ul-Haq
Zafar, Yusuf
Keywords: Gossypium hirsutum L, callus induction, somatic embryogenesis, nitrogen sources, plant regeneration
Issue Date: 31-Dec-2004
Publisher: Academic Journals
Citation: African Journal of Biotechnology (ISSN: 1684-5315) Vol 3 Num 6
Abstract: Cotton (Gossypium hirsutum   L.) cv Coker-312 callus culture was assessed in terms of its usefulness as a system for investigating the effect of nitrates from different chemical compounds of nitrogen on embryo induction percentage in calli as the plant growth and cell differentiation mainly based on nitrogen. Both sources and amount of nitrogen in in vitro medium have significant effects mainly on cell growth, embryogenesis and the production of anthocyanin. Anthocyanin production is the best indication of inhibition of cell growth in in vitro culture of cotton. Embryo induction rate was high when NH4NO3 was eliminated from the medium but in the presence of KNO3. The dicotyledenary embryos were developed with in 5 weeks, these embryos developed into normal plantlets immediately when they were cultured on a simple MS basal medium supplemented with 3% glucose.
URI: http://hdl.handle.net/1807/6503
Other Identifiers: http://www.bioline.org.br/abstract?id=jb04062
Rights: Copyright 2004 - Academic Journals.
Appears in Collections:Bioline International Legacy Collection

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