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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/6555

Title: Studies on improved Agrobacterium-mediated transformation in two indica rice (Oryza sativa   L.)
Authors: Saharan, Vinod
Yadav, Ram C.
Yadav, Neelam R.
Ram, Khushi
Keywords: Agrobacterium, indica rice, transformation, acetosyringone.
Issue Date: 31-Dec-2004
Publisher: Academic Journals
Citation: African Journal of Biotechnology (ISSN: 1684-5315) Vol 3 Num 11
Abstract: Agrobacterium tumefaciens   strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR-126. High concentrations of acetosyringone in the Agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transformation studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR-46 (28.9%). The percent recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 (52.6%).
URI: http://hdl.handle.net/1807/6555
Other Identifiers: http://www.bioline.org.br/abstract?id=jb04114
Rights: Copyright 2004 - Academic Journals
Appears in Collections:Bioline International Legacy Collection

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