|Title: ||Studies on improved Agrobacterium-mediated transformation in two indica
rice (Oryza sativa L.)|
|Authors: ||Saharan, Vinod|
Yadav, Ram C.
Yadav, Neelam R.
|Keywords: ||Agrobacterium, indica rice, transformation, acetosyringone.|
|Issue Date: ||31-Dec-2004|
|Publisher: ||Academic Journals|
|Citation: ||African Journal of Biotechnology (ISSN: 1684-5315) Vol 3 Num 11|
|Abstract: ||Agrobacterium tumefaciens strain EHA 105 carrying binary
vector pCAMBIA 1301 was used for transformation in two economically
important highly recalcitrant indica rice cultivars HKR-46 and HKR-126.
High concentrations of acetosyringone in the Agrobacterium culture and
co-cultivation medium proved to be indispensable for successful
transformation. Embryogenic scutellar calli were used for
transformation studies. Binary vector pCAMBIA 1301 have been proved
efficient for transformation. The percent transient GUS expression
found to be higher in cutivar HKR-126 (44.4%) as compared to HKR-46
(28.9%). The percent recovery of hygromycin resistant calli after 4-6
weeks on selection medium was maximum in HKR-126 (52.6%).|
|Other Identifiers: ||http://www.bioline.org.br/abstract?id=jb04114|
|Rights: ||Copyright 2004 - Academic Journals|
|Appears in Collections:||Bioline International Legacy Collection|