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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/669

Title: Identification of growth hormone in the sea lamprey, an extant representative of a group of the most ancient vertebrates
Authors: Kawauchi, Hiroshi
Suzuki, Kunimasa
Yamazaki, Tomohide
Moriyama, Shunsuke
Nozaki, Masumi
Yamaguchi, Kiyoko
Takahashi, Akiyoshi
Youson, John
Sower, Stacia
Issue Date: 2002
Publisher: The Endocrine Society
Citation: Endocrinology 143: 4916-4921, 2002.
Abstract: GH was identified in the sea lamprey, an extant representative of a group of the most ancient vertebrates, the Agnatha. A putative GH-cDNA was cloned from the pituitary by RT-PCR. The entire coding region comprised an open-reading frame of 203 amino acids (aa). The mature protein was also isolated from pituitaries, and fractionated by gel filtration and reverse-phase HPLC. A putative GH was monitored by Western blotting with a rabbit antiserum against a synthetic peptide corresponding to pre-GH sequence (aa 29–45). Sequence analysis of the purified protein demonstrated that the prehormone consists of a signal peptide of 22 aa and the mature protein of 181 aa, which shows 25% sequence identity with sturgeon GH. The site of production was identified through immunohistochemistry to be cells of the dorsal half of the proximal pars distalis of the pituitary. Following cDNA cloning of lamprey IGF cDNA, it was shown using RT-PCR that lamprey GH stimulates IGF expression in lamprey liver. This is the first study in which a member of the GH/prolactin/somatolactin family has been identified in an agnathan. In addition, GH appears to be the only member of this hormone family in the sea lamprey. Evidence suggests that GH is the ancestral hormone in the molecular evolution of the GH family and that the endocrine mechanism for growth stimulation was established at an early stage of vertebrate evolution.
URI: http://hdl.handle.net/1807/669
Appears in Collections:Biology

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