|Title: ||Further screening of Venda medicinal plants for activity against HIV
type 1 reverse transcriptase and integrase|
|Authors: ||Bessong, Pascal O.|
Rojas, Luis B.
Obi, Larry C.
Tshisikawe, Peter M.
Igunbor, Eunice O.
|Keywords: ||HIV-1; reverse transcriptase; integrase; medicinal plants; inhibition;
Venda; South Africa|
|Issue Date: ||31-Dec-2006|
|Publisher: ||Academic Journals|
|Citation: ||African Journal of Biotechnology (ISSN: 1684-5315) Vol 5 Num 6|
|Abstract: ||The use of medicinal plants for AIDS-related conditions is common in
South Africa. In order to establish an antiviral rationale for the use
of these plants we screened fractions of the methanol extracts of
medicinal plants for activity against HIV-1 reverse transcriptase (RT)
and integrase (IN). The n-butanol fraction obtained from the crude
methanol extracts of the roots of Bridelia micrantha (Hochst)
Baill. (Euphorbiaceae) was observed to be as the most active inhibiting
the RNA-dependent-DNA polymerization (RDDP) activity of HIV-1 RT with
an IC50 of 7.3 μg/ml. However, it had no activity on the 3'-end
processing activity of HIV integrase. Bioassay-guided fractionation of
the n-butanol fraction yielded friedelin and β-sistosterol, which
did not inhibit the RDDP of RT or 3'-end processing functions of IN
even at a concentration of 500 μM. An uncharacterized fraction
obtained in the bioassay-guided fractionating process inhibited the
RDDP with an IC50 of 9.6 μg/ml, but had no inhibition on IN.
Phytochemical screening indicated the presence of flavonoids and
tannins in the uncharacterized fraction.|
|Other Identifiers: ||http://www.bioline.org.br/abstract?id=jb06092|
|Rights: ||Copyright 2006 - Academic Journals|
|Appears in Collections:||Bioline International Legacy Collection|