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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/6731

Title: DGGE-RAPD analysis as a useful tool for cultivar identification
Authors: Bahieldin, A.
Ahmed, I. A.
Gad El-Karim, Gh.A.
Eissa, H. F.
Mahfouz, H. T.
Saleh, O. M.
Keywords: Denaturing gradient gel electrophoresis-random amplified polymorphic DNA, artificial heteroduplex, dendogram, lineage.
Issue Date: 31-Dec-2006
Publisher: Academic Journals
Citation: African Journal of Biotechnology (ISSN: 1684-5315) Vol 5 Num 8
Abstract: Denaturing gradient gel electrophoresis-random amplified polymorphic DNA (DGGE-RAPD) was used to overcome the main drawbacks of RAPD (i.e., the low levels of reproducibility and polymorphism). As a model, six barley cultivars of known origin were tested for RAPD markers using DGGE methodology with 29 arbitrary primers. Among a total of 418 bands observed, as high as 99 were polymorphic. Comparison between agarose-RAPD and DGGE-RAPD revealed that the latter was highly reproducible and gave higher level of polymorphism and consequently more markers. The relationships among barley cultivars derived from this study based on DGGE-RAPD are consistent with the known lineage of these cultivars. In conclusion, we recommend the use of DGGE-RAPD as an alternative tool to the more costly DNA-based analysis in cultivar identification in laboratories with limited funds.
URI: http://hdl.handle.net/1807/6731
Other Identifiers: http://www.bioline.org.br/abstract?id=jb06101
Rights: Copyright 2006 - Academic Journals
Appears in Collections:Bioline International Legacy Collection

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