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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/6844

Title: Gel purified lipl32: a prospective antigen for detection of leptospirosis
Authors: Tahiliani, P.
Kumar, M. Mohan
Chandu, D.
Kumar, A.
Nagaraj, C.
Nandi, D.
Keywords: Leptospira, LipL32, Human IgG ELISA, Gel elution
Issue Date: 31-Dec-2005
Publisher: Medknow Publications and Staff Society of Seth GS Medical College and KEM Hospital, Mumbai, India
Citation: Journal of Postgraduate Medicine (ISSN: 0972-2823) Vol 51 Num 3
Abstract: Background: Leptospirosis, a zoonosis, is a re-emerging disease, affecting populations across the globe. However, the current methods of diagnosis are time- consuming, cumbersome, imprecise or expensive. Aim: To develop an assay for differential and early diagnosis of Leptospirosis. Methods and Material: IgG based ELISA for evaluation of three antigens, namely, a gel-purified recombinant protein (rLipL32), secreted proteins and whole organism sonicates of Leptospira spp. The antigens were evaluated using, rabbit polyclonal antiserum and human sera samples. Results: Studies with a rabbit polyclonal antiserum indicated the utility of these antigens in differentiating Leptospira from other common pathogenic organisms. Evaluation of these antigens with fifteen representative human serum samples indicated gel-purified rLipL32 to be a potentially useful antigen for detection of leptospirosis. The results obtained with IgG ELISA were correlated with the results of microscopic agglutination test (MAT). Conclusion: Gel-purified rLipL32 is a valuable antigen for early and accurate diagnosis of leptospirosis. Further evaluation of this assay in field conditions and larger sera samples will indicate its suitability in case of an epidemic.
URI: http://hdl.handle.net/1807/6844
Other Identifiers: http://www.bioline.org.br/abstract?id=jp05066
Rights: Copyright 2005 Journal of Postgraduate Medicine.
Appears in Collections:Bioline International Legacy Collection

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