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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8044

Title: Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil
Authors: Mendes dos Santos, Joselita Maria
Maia, Juracy de Freitas
Tadei, Wanderli Pedro
Rodriguez, Gloria Alícia Diaz
Keywords: Nyssorhynchus - Anopheles - genetic variability - isozymes - Amazon - Brazil
Issue Date: 31-Dec-2003
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 98 Num 2
Abstract: An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi , An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic intere specific divergence. Samples from Macap á , State of Amap á and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi , An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters.
URI: http://hdl.handle.net/1807/8044
Other Identifiers: http://www.bioline.org.br/abstract?id=oc03063
Rights: Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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