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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/81

Title: Use of green fluorescent protein for online, single cell detection of bacteria introduced into activated sludge microcosms
Authors: Eberal, Leo
Ammendola, Aldo
Geisenberger, Otto
Schulze, Renate
Givskov, Michael
Sternberg, Claus
Molin, S. Ren
Schleifer, K. H.
Keywords: green fluorescent protein, in situ hybridisation, protozoan grazing, activated sludge
Issue Date: Feb-1998
Publisher: Biostrategy Associates
Citation: Biofilm Journal 3(1)
Abstract: A derivative of broad host-range plasmid RP4 was tagged with the green fluorescent protein (GFP) and this construct was used to mark various Gram-negative bacteria. Video recording of combined phase contrast and epifluorescence microscopy was employed to track the fate of individual marked cells after introduction into a sludge microcosm. Whether the introduced bacteria were laboratory strains or activated sludge isolates. they were found to be rapidly eliminated from the microcosms as a result of intense predation by protozoa. Furthermore, a method was developed that allows detection of GFP-tagged bacteria in fixed samples that are simultaneously used for in situ hybridisation with a eukaryote-specific rRNA-targeted oligonucleotide probe for visualisation of protozoa.
URI: http://hdl.handle.net/1807/81
Appears in Collections:Bioline International Legacy Collection

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