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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8122

Title: Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Strongyloidiasis
Authors: Silva, Luciana Pereira
Solange da Costa Barcelos, Ivanildes
Passos-Lima, Andréia Barcelos
Espindola, Foued Salmen
Campos, Dulcinéa Maria Barbosa
Costa-Cruz, Julia Maria
Keywords: Strongyloides stercoralis - Strongyloides ratti - strongyloidiasis - immunodiagnosis - Western blotting
Issue Date: 31-Dec-2003
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 98 Num 5
Abstract: The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology.
URI: http://hdl.handle.net/1807/8122
Other Identifiers: http://www.bioline.org.br/abstract?id=oc03141
Rights: Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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