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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8150

Title: Cytotoxicity and potential antiviral evaluation of violacein produced by Chromobacterium violaceum  
Authors: Andrighetti-Fröhner, C. R.
Antonio, R. V.
Creczynski-Pasa, T. B.
Barardi, C. R. M.
Simões, C. M. O.
Keywords: violacein - cytotoxicity - antiviral - MTT assay - herpes simplex virus type 1- poliovirus - rotavirus - hepatitis A virus - adenovirus
Issue Date: 31-Dec-2003
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 98 Num 6
Abstract: Natural products are an inexhaustible source of compounds with promising pharmacological activities including antiviral action. Violacein, the major pigment produced by Chromobacterium violaceum, has been shown to have antibiotic, antitumoral and anti-Trypanosoma cruzi   activities. The goal of the present work was to evaluate the cytotoxicity of violacein and also its potential antiviral properties.The cytotoxicity of violacein was investigated by three methods: cell morphology evaluation by inverted light microscopy and cell viability tests using the Trypan blue dye exclusion method and the MTT assay. The cytotoxic concentration values which cause destruction in 50% of the monolayer cells (CC50) were different depending on the sensitivity of the method. CC50 values were ≥ 2.07 ± 0.08 μM for FRhK-4 cells: ≥ 2.23 ± 0.11 μM for Vero cells; ≥ 2.54 ± 0.18 μM for MA104 cells; and ≥ 2.70 ± 0.20 μM for HEp-2 cells. Violacein showed no cytopathic inhibition of the following viruses: herpes simplex virus type 1 (HSV-1) strain 29-R/acyclovir resistant, hepatitis A virus (strains HM175 and HAF-203) and adenovirus type 5 nor did it show any antiviral activity in the MTT assay. However violacein did show a weak inhibition of viral replication: 1.42 ± 0.68%, 14.48 ± 5.06% and 21.47 ± 3.74% for HSV-1 (strain KOS); 5.96 ± 2.51%, 8.75 ± 3.08% and 17.75 ± 5.19% for HSV-1 (strain ATCC/VR-733); 5.13 ± 2.38 %, 8.18 ± 1.11% and 8.51 ± 1.94% for poliovirus type 2; 8.30 ± 4.24%; 13.33 ± 4.66% and 24.27 ± 2.18% for simian rotavirus SA11, at 0.312, 0.625 and 1.250 mM, respectively, when measured by the MTT assay.
URI: http://hdl.handle.net/1807/8150
Other Identifiers: http://www.bioline.org.br/abstract?id=oc03169
Rights: Copyright 2003 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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