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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8250

Title: Isolation and characterization of the full-length cDNA encoding a member of a novel cytochrome p450 family (CYP320A1) from the tropical freshwater snail, Biomphalaria glabrata, intermediate host for Schistosoma mansoni
Authors: Lockyer, Anne E.
Noble, Leslie R.
Rollinson, David
Jones, Catherine S.
Keywords: Simulium (Psaroniocompsa) - polytene chromosomes - cytotaxonomy - Brazilian Amazon
Issue Date: 31-Dec-2005
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 100 Num 3
Abstract: Cytochrome p450s (cyp450s) are a family of structurally related proteins, with diverse functions, including steroid synthesis and breakdown of toxins. This paper reports the full-length sequence of a novel cyp450 gene, the first to be isolated from the tropical freshwater snail Biomphalaria glabrata , an important intermediate host of Schistosoma mansoni . The nucleotide sequence is 2291 bp with a predicted amino acid sequence of 584aa. The sequence demonstrates conserved cyp450 structural motifs, but is sufficiently different from previously reported cyp450 sequences to be given a new classification, CYP320A1. Initially identified as down-regulated in partially resistant snails in response to S. mansoni infection, amplification of this gene using RT-PCR in both totally resistant or susceptible snail lines when exposed to infection, and all tissues examined, suggests ubiquitous expression. Characterization of the first cyp450 from B. glabrata is significant in understanding the evolution of these metabolically important proteins.
URI: http://hdl.handle.net/1807/8250
Other Identifiers: http://www.bioline.org.br/abstract?id=oc05079
Rights: Copyright 2005 Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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