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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8264

Title: Lymphocyte subset analyses in healthy adults vaccinated with yellow fever 17DD virus
Authors: Paula dos Santos, Ana
Bertho, Álvaro Luiz
Dias, Daniela Capuzzo
Santos, Jaciara Ramos
Marcovistz, Rugimar
Keywords: yellow fever - vaccine - flow cytometry - neutralizing antibodies - lymphocyte subsets
Issue Date: 31-Dec-2005
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 100 Num 3
Abstract: In this study the kinetics of humoral and cellular immune responses in first-time vaccinees and re-vaccinees with the yellow fever 17DD vaccine virus was analyzed. Flow cytometric analyses were used to determine percentual values of T and B cells in parallel to the yellow fever neutralizing antibody production. All lymphocyte subsets analyzed were augmented around the 30th post vaccination day, both for first-time vaccinees and re-vaccinees. CD3+ T cells increased from 30.8% (SE ± 4%) to 61.15% (SE ± 4.2%), CD4+ T cells from 22.4% (SE ± 3.6%) to 39.17% (SE ± 2%) with 43% of these cells corresponding to CD4+CD45RO+ T cells, CD8+ T cells from 15.2% (SE ± 2.9%) to 27% (SE ± 3%) with 70% corresponding to CD8+CD45RO+ T cells in first-time vaccinees. In re-vaccinees, the CD3+ T cells increased from 50.7% (SE ± 3%) to 80% (SE ± 2.3%), CD4+ T cells from 24.9% (SE ± 1.4%) to 40% (SE ± 3%) presenting a percentage of 95% CD4+CD45RO+ T cells, CD8+ T cells from 19.7% (SE ± 1.8%) to 25% (SE ± 2%). Among CD8+CD38+ T cells there could be observed an increase from 15 to 41.6% in first-time vaccinees and 20.7 to 62.6% in re-vaccinees. Regarding neutralizing antibodies, the re-vaccinees presented high titers even before re-vaccination. The levels of neutralizing antibodies of first-time vaccinees were similar to those presented by re-vaccinees at day 30 after vaccination, indicating the success of primary vaccination.Our data provide a basis for further studies on immunological behavior of the YF 17DD vaccine.
URI: http://hdl.handle.net/1807/8264
Other Identifiers: http://www.bioline.org.br/abstract?id=oc05093
Rights: Copyright 2005 Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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