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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8299

Title: Resistance to reinfection in rats induced by irradiated metacercariae of Clonorchis sinensis  
Authors: Quan, Fu Shi
Lee, Jeong Beom
Bae, Jun Sang
Ohwatari, Nobu
Min, Young Ki
Yang, Hun Mo
Keywords: irradiation - metacercariae - reinfection - interferon-g - interlukin-2
Issue Date: 31-Dec-2005
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 100 Num 5
Abstract: A study was made to observe the association between the resistance to reinfection induced by irradiated metacercariae (MC) of Clonorchis sinensis   and antigen specific Th1- and Th2-type cytokine productions in rats. Rats were infected with 20 MC of C. sinensis, previously exposed to a single dose of gamma irradiation, which varied from 0 to 100 Gy. All of them, single dose of 12 Gy showed higher IgG antibody titer with lowest worm recovery. Thus, 50 MC were used to challenge infection in rats previously infected with 20 MC irradiated at 12 Gy and the highest resistance to challenge infection was observed. The results of lymphocyte proliferation with specific antigen, ES Ag were shown no difference of proliferative responses as compared with primary and challenge infection at 12 Gy irradiation dose. In the case of cytokines production were observed that interferon (IFN-γ) and interlukin (IL-2) were significantly enhanced, while IL-4 and IL-10 was almost unchanged to make comparison between primary and secondary infection at 12 Gy irradiation dose. In conclusion, the single dose of 12 Gy could be adopted for induction of the highest resistance to challenge infection. Up-regulation of Th1 type cytokines, IFN-γ and IL-2 may be affected to develop vaccine by irradiated MC.
URI: http://hdl.handle.net/1807/8299
Other Identifiers: http://www.bioline.org.br/abstract?id=oc05128
Rights: Copyright 2005 - Instituto Oswaldo Cruz - Fiocruz.
Appears in Collections:Bioline International Legacy Collection

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