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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/8328

Title: Mycobacterium avium restriction fragment lenght polymorphism- IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
Authors: Carvalho de Sequeira, Patrícia
Fonseca, Leila de Souza
Gomes da Silva, Marlei
Saad, Maria Helena Féres
Keywords: IS1254-restriction fragment length polymorphism - Mycobacterium avium - MaDRE-polymerase chain reaction - nontuberculous mycobacteria - AIDS
Issue Date: 31-Dec-2005
Publisher: Fundação Oswaldo Cruz, Fiocruz
Citation: Memórias do Instituto Oswaldo Cruz (ISSN: 1678-8060) Vol 100 Num 7
Abstract: Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.
URI: http://hdl.handle.net/1807/8328
Other Identifiers: http://www.bioline.org.br/abstract?id=oc05157
Rights: Copyright 2005 - Instituto Oswaldo Cruz - Fiocruz
Appears in Collections:Bioline International Legacy Collection

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