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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/85

Title: Susceptibility of biofilms of Streptococccus sanguis and Actinomyces naeslundii to chlorhexidine
Authors: Larsen, Tove
Stoltze, Kaj
Fiehn, Nils-Erik
Keywords: biofilm, antimicrobial susceptibility, chlorhexidine, Streptococcus sanquis, Actinomyces naeslundii
Issue Date: Jul-1997
Publisher: Biostrategy Associates
Citation: Biofilm Journal 2(1)
Abstract: Single species and two species biofilms comprising Streptococcus sanquis and Actinomyces naeslundii were established in order to determine the susceptibility to chlorhexidine of the bacteria growing in biofilms compared to planktonic growth, and when growing in single species biofilms compared to mixed biofilms. The MIC of the bacteria to chlorhexidine was determined by a broth dilution method. Single and two species biofilms were established in a flow model in a modified Robbins device and subsequently exposed to chlorhexidine for 48 h at concentrations from 10 to 2000 mg/ml. Further, two species biofilms were exposed to 1000 mg/ml of chlorhexidine in 10 min pulses two or six times a day. The biofilm cell number was determined by viable counting at different time intervals. Two species biofilms were established faster than single species biofilms, but after establishment A. naeslundii was suppressed by S. sanguis. When lower concentrations of chlorhexidine were added, however, A. naeslundii also remained in the biofilm. Resistance of S. sanguis and A. naeslundii to chlorhexidine increased considerably when growing in biofilms and >100 mg/ml was needed to eliminate growth of the bacteria. Somewhat different patterns of susceptibility were observed in single species compared to two species biofilms. Thus, the results of this study indicate that the susceptibility of bacteria to antimicrobial agents is influenced by biofilm growth as well as by bacterial interactions in mixed bacterial communities.
URI: http://hdl.handle.net/1807/85
Appears in Collections:Bioline International Legacy Collection

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